Library
Each entry denotes one library, even if multiple aliquots are made and sequenced at different times. A library means something with Illumina sequencing primers, indices or barcodes as needed, and ready to pool and put into a lane.
| Name | Type | Required |
|---|---|---|
| s2cDNASampleNumber | int | Yes (Foreign Key) |
| libraryDate | datetime | Yes (Primary Key) |
| libraryPreparer | string | Yes |
| libraryObservations | string | Yes |
| index1Sequence | string | Yes |
| libraryProtocol | string | Yes |
| index1Name | string | Yes |
| index2Name | string | Yes |
| index2Sequence | string | Yes |
| roboticLibraryPrep | boolean | Yes |
s2cDNASampleNumber
Foreign key to s2 table
libraryDate
The date (e.g. 05.17.20) on which the sequencing library was prepared and ready for use. Provide the date when the library was completed. See general specifications for format details.
libraryPreparer
Name (e.g. M.BRENT) of the person who was in charge of the sequencing library preparation. If more than one person was involved, just pick one. See general specifications for format details.
librarySampleNumber
Sequential positive integers uniquely identifying the libraries prepared by a given preparer on a given date.
index1Name
E.g. “Index1_2”.
index1Sequence
The sequence of index one (all caps), used to help distinguish samples pooled into a single lane. “NA” if no indices are used.
index2Name
- SIC_Index2_06
- universal
index2Sequence
The sequence of index two (all caps), used to help distinguish samples pooled into a single lane. “NA” if zero or one indices are used.
libraryProtocol
Use only the first part of the descrition below, eg E7420L. Datatype must be a single string of letters with no whitespace.
- SolexaPrep - homebrew kit used prior to 10-25-2015 (best current estimate)
- E7420L - NEBNext Ultra Directional RNA Library Prep Kit for Illumina
- If a concentration other than typical is used, list it as such: E7420L_0.5X
- E7760L - NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
roboticLibraryPrep
“True” (with that capitalization) if the robot was used, “False” if not.