S1cdnaSample

First Strand cDNA sample Table

If multiple aliquots of the first strand product made in a single pool or well are combined into different pools for second strand synthesis, they are the same first strand sample so only one entry is needed.

Name	Type	Required
rnaSampleNumber	int	yes (Foreign Key)es
s1cDNADate	datetime	yes (Primary Key)
s1cDNAPreparer	string	Yes
s1Observations	string	Yes
polyAIsolationProtocol	string	Yes
s1cDNAProtocol	string	Yes
roboticS1Prep	boolean	Yes
s1PrimerSeq	string	Yes

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rnaSampleNumber

[REQUIRED] Foreign key to rnaSample Table

s1cDNADate

[REQUIRED] The date (e.g. 05.17.20) on which the first strand cDNA was prepared and ready for use. See date format at the top of this document. See general specifications for format details.

s1cDNAPreparer

[REQUIRED] Name of the person (e.g. M.BRENT) who was in charge of the first strand cDNA preparation (try not to split these, or we will have to add more fields!). If more than one person was involved, just pick one. See general specifications for format details.

s1cDNASampleNumber

[REQUIRED] Sequential positive integers uniquely identifying the single-stranded cDNA or cDNA-RNA hybrid samples prepared by a given preparer on a given date.

polyAIsolationProtocol

[REQUIRED] Entries, to be defined, indicating the kit or protocol used for isolating polyA+ rna from total RNA or for depleting unwanted RNAs such as ribosomal RNAs. Should be a single string of letters with no whitespace. Add any entries used below, with an explanation.

• E7490L -- NEBNext Poly(A) mRNA Magnetic Isolation Module, thermo catalog number E7490
• catcher -- mRNA Catcher Plus
• None -- if no polyA isolation was performed, as in BRBSeq

s1cDNAProtocol

[REQUIRED] Entries, to be defined, indicating the kit or protocol used for generating the first strand. Should be a single string of letters with no whitespace. Add any entries used below, with an explanation.

• SuperScriptIII
• E7420L - NEBNext Ultra Directional RNA Library Prep Kit for Illumina
• If a concentration other than typical is used, list it as such: E7420L_0.5X
• E7760L - NEBNext Ultra II Directional RNA Library Prep Kit for Illumina

roboticS1Prep

[REQUIRED] Boolean. Make sure formatting of True/False value is appropriate for the program you are using

s1PrimerSeq

[REQUIRED] The sequence (all caps) of the primer if an indexed primer is being used, or “random” if random primers are used.