RnaSample
If multiple aliquots of RNA are made from a single tube or well, they are the same RNA sample, so only one entry is needed.
| Name | Type | Required |
|---|---|---|
| bioSampleNumber | int | yes (Foreign Key) |
| rnaSampleNumber | string | yes (Primary Key) |
| rnaDate | datetime | Yes |
| rnaPreparer | string | Yes |
| rnaSampleObservations | string | Yes |
| rnaPrepMethod | string | Yes |
| roboticRnaPrep | boolean | Yes |
| ribosomalBand | boolean | No |
| ribosomalBandShape | string | No |
| smallRNABands | string | No |
| rin | float | No |
bioSampleNumber
Foreign key to bioSample Table
rnaSampleNumber
Primary key of rnaSample table
rnaDate
The date (e.g. 05.17.20) on which the total rna was prepared and ready for use. See general specifications for format details.
rnaPreparer
Name of the person (e.g. M.BRENT) who was in charge of the total RNA isolation. If more than one person was involved, just pick one. See general specifications for format details.
rnaSampleObservations
[OPTIONAL] This is a free text entry field meant to record anything about this sample, specifically pertaining to this table's data, that does not fit into other fields. This should not be used for storing any information pertinent to experimental design.
rnaPrepMethod
One of the following. If anything other than these is entered, it should be added to the list below with an explanation. For each entry, there should be a detailed protocol document with the same name. Any time the protocol is changed, a new document should be made up describing the differences relative to an older document. Older documents should never be deleted.
- DirectZol -- DirectZol kit from Zymo, per instructions
- RiboPure0.5X -- RiboPure Yeast kit from Ambion, using 0.5 times volume and reagents in instructions.
- RiboPure0.25X -- RiboPure Yeast kit from Ambion, using 0.25 times volume and reagents in instructions.
- RiboPure0.125X -- RiboPure Yeast kit from Ambion, using 0.125 times volume and reagents in instructions.
- ComboA -- uses a combination of RNA prep methods, homogenize RNA following the Directzol kit from Zymo, and purify RNA using the RiboPure Yeast kit from Ambion (using 0.25 times volume and reagents).
- ComboB -- uses a combination of RNA prep methods, homogenize RNA following the RiboPure Yeast kit from Ambion (using 0.25 times volume and reagents), and purify RNA using the Directzol kit from Zymo.
- TRIzol
rnaPrepProtocol
The name of a file in the protocols directory under the experimenter directory. Can be reused in multiple files -- no need to rename as long as the protocol is unchanged.
roboticRnaPrep
[REQUIRED] Boolean, True of False (be sure to use the correct formatting for whatever program you're using for this)
ribosomalBand (TRUE or FALSE)
[OPTIONAL] Boolean, True of False (be sure to use the correct formatting for whatever program you're using for this)
ribosomalBandShape
[OPTIONAL] ChoiceS: straight, smile, or NA
smallRNABands
[OPTIONAL] Boolean, True of False (be sure to use the correct formatting for whatever program you're using for this)
rin
[OPTIONAL] A value assigned by the Bioanalyzer between 0 and 10