S2cdnaSample
Second Strand cDNA Table
There should be one entry per tube full of double-stranded DNA.
| Name | Type | Required |
|---|---|---|
| s1cDNASampleNumber | int | Yes |
| s2cDNADate | datetime | Yes |
| s2cDNAPreparer | string | Yes |
| s2Observations | string | Yes |
| roboticS2Prep | boolean | Yes |
| PooledSecondStrand | boolean | Yes |
s1cDNADate, s1cDNAPreparer, S1cDNASampleNumber
[REQUIRED] The entries in these three columns should exactly match the corresponding entries for one and only row of a s1cDNASample table. See general specifications for format details.
s2cDNADate
[REQUIRED] The date (e.g. 05.17.20) on which the second strand cDNA was prepared and ready for use. See general specifications for format details.
s2cDNAPreparer
[REQUIRED] Name (e.g. M.BRENT) of the person who was in charge of the second strand cDNA preparation (try not to split these, or we will have to add more fields!). If more than one person was involved, just pick one. See general specifications for format details.
s2cDNASampleNumber
[REQUIRED] Sequential positive integers uniquely identifying the double-stranded cDNA samples prepared by a given preparer on a given date.
s2cDNAProtocol
[REQUIRED] Entries, to be defined, indicating the kit or protocol used for generating the second strand. Should be a single string of letters with no whitespace. Add any entries used below, with an explanation.
- SuperScriptIII
- E7420L - NEBNext Ultra Directional RNA Library Prep Kit for Illumina
- If a concentration other than typical is used, list it as such: E7420L_0.5X
- E7760L - NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
PooledSecondStrand
[REQUIRED] Boolean. Make sure formatting of True/False value is appropriate for the program you are using
roboticS2Prep
[REQUIRED] Boolean. Make sure formatting of True/False value is appropriate for the program you are using